SELECTIVE TOXICITY OF RHODAMINE 123 ON CARCINOMA CELL IN VITRO
- VernacularTitle:Rh-123对体外肿瘤细胞选择性毒性作用
- Author:
Jie ZHONG
;
Jingbo ZHANG
- Publication Type:Journal Article
- Keywords:
Selective toxicity;
HeLa cell;
Mitochondria;
Rhodamine 123;
Enzyme cytochemistry stain;
Electron microscopy
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The selective toxicity of the mitochondria-specific cationic fluorescent dye rhodamine 123 (Rh-123) on HeLa cells in culture was studied. In this report, we demonstrate that with continuous exposure, Rh-123 markedly inhibited the growth of HeLa cells but had little effect on normal human kidney fibroblasts. With continuously exposed to Rh-123, the growth rate, colony forming ability, and mitotic index of HeLa cells were decreased. The mechanism of toxicity of Rh-123 on HeLa ceils was investigated by EM and enzyme cytochemistry stain. The mitochondria of carcinoma cells were the main targets for the inhibitory action of Rh-123, since they selectively accumulated the dye. At the dosage of Rh-123 which was toxic to HeLa cells, the structure and function of mitochondria were disrupted, as the mitochondriarelated enzymes, i. e., ATPase, LDH, and SDH were inhibited. The possible mechanism of the action of Rh-123 on HeLa cells is briefly discussed.
