LOCALIZATION AND QUANTITATION OF ESTROGEN RECEPTORS IN SEVERAL TISSUES OF DIFFERENT STRAINS OF MICE BY AUTORADIOGRAPHY
- VernacularTitle:雌激素受体在不同品系小鼠组织中的放射自显影定位和定量研究
- Author:
Fengming GAO
;
Zhi ZHANG
- Publication Type:Journal Article
- Keywords:
Estrogen receptors;
Tritiated estradiol;
Tumor induction;
Autoradiography;
Mice
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The distributions of estrogen receptors in preoptic region of hypothalamus, anterior lobe of pituitary gland, mammary gland, thymus and liver of LACA and C57 BL strains of mice were studied by ~3H-estradiol and autoradiography in frozen sections.In both strains of mice, labelling index (LI) was more than 85％ in preoptic region of hypothalamus and anterior lobe of pituitary gland, and average number of silver grains (AG) was more than 19. LI and AG of epithelial cells of mammary glands in LACA strain of mice were quite high, 96％ and 12 respectively; but in C57BL strain of mice were only 5％ and 5 respectively. A few hepatic cells and lymphocytes of thymus were labelled.According to the above results the reason of why mammary carcinoma of female LACA strain of mice and adenoma of pituitary gland of C57BL strain of mice could 15e easily induced by estrogenic hormones (estradiol valerate and hydroxyprogesterone caproate, EP), but no mammary carcinoma was induced in C57BL strain of mice by EP, and this can be partly explained by the presence of a large or few number of estrogen receptors. However, the presence of estrogen receptors was only a prerequisite but not a unique condition. Other factors should be considered, because many more estrogen receptors were present in preoptic region of hypothalamus of both strains of mice and anterior lobe of pituitary gland of LACA strain of mice, but few or no tumors could be induced by EP.
