GERL IN FETAL HEPATOCYTES: AN ULTRASTRUCTURE AND ELECTRON MICROSCOPIC HISTOCHEMISTRY STUDY ON THE RAT FETUSES
- VernacularTitle:大白鼠胎肝细胞GERL的超微结构和电镜细胞化学观察
- Author:
Li WU
;
Qimin WANG
- Publication Type:Journal Article
- Keywords:
GERL;
AcP;
Ultrastructure;
Hepatocyte;
Fetuses;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
GERL in fetal hepatocytes of 12, 15, 17, 19 and 21 days Wistar rat fetuses was investigated by means of transmission electron microscopy and ultracytochemistry. The ultrastructural study showed that: on the day 12 fetuses, GERL of fetal hepatoeytes was consisted of rigid lamellae and short tubules. Rigid lamellae were characterized by the relatively rigid, thin tubules and located at the maturation face of Golgi apparatus. On the day 15 and 19 fetuses, GERL was composed of tubulevacuole-like rigid lamellae, some vesicles, short or thick tubules and round dence bodies. GERL was often found tobe contiuned with lysosomes, including coated vesicle, primary and secondary lysosomes. On the day 17 and 21 fetuses, when the structure of fetal hepatocytes was at the stage of differentiation and renewal, GERL degenerated and could notbe identified.Acid phosphatase ultraeytochemieal results showed that the enzyme reaction product was seen in all parts of GERL and lysosomes, however, no portion of the Golgi stack showed AcP activity. AcP reaction product could also demons trated in the network form and degenerated form of GERL.As mentioned above, our study suggested: (1) there was indeed GERL in fetal hepatocytes of rat fetuses; (2) the formation of GERL was related to the differentiation of fetal hepatocytes as well as the formation and function of lysosomes; (3) according to the unique ultrastructure, enzymological and functional features, it be a preference to the view that the GERL might be a distinct cytoplasmic organelle.
