THE OPTIMAL CONDITION OF THE STAINING PROCEDURE USING MONOCLONAL ANTIBODY TO BROMODEOXYURIDINE (BRDURD)
- VernacularTitle:抗溴化脱氧尿嘧啶核苷单克隆抗体免疫荧光细胞化学染色过程的最佳条件实验研究
- Author:
Xuegeng SHI
;
Nishiya IWAO
;
Yoshizumi NOBORU
- Publication Type:Journal Article
- Keywords:
Bromodeoxyuridine;
Monoclonal antibody;
DNA partial denaturation;
Dual-parameter;
Immunofluorescence;
Cell cycle;
Flow cytometry
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The staining procedure using the monoclonal antibody to BrdUrd involved the following important steps: the duration of BrdUrd labeling time, the concentration of HC1, the duration of HCI treatment for cellular DNA partial denaturation and so on. The ratio of mean intensities between BrdUrd positive cells and BrdUrd negative cells, the proportion of cell aggregation occuring during HCI treatment, the relative fluorescence intensity and coefficient of variation (CV) of G_1 peak were considered as the criteria of optimal conditions of the whole staining procedure. The optimal results of this staining procedure were obtained under the conditions of 30 rain BrdUrd labeling time, 2.4 mol/L HC1, 30 min HC1 treatment, 1 hour incubation of the monoclonal antibody to BrdUrd and without RNase treatment. With this staining procedure, the optimal staining results were obtained for KF-1, KFr, HeLa and IK-90 cell lines.
