PREPARATION OF ~(35)S-LABEJJED CPS_1 cDNA PROBE FOR IN SITU HYBRIDIZATION
- VernacularTitle:制备~(35)S标记的氨甲酰磷酸合成酶I的cDNA探针用于原位分子杂交
- Author:
Yiping YANG
;
Shie LI
;
Lian DING
- Publication Type:Journal Article
- Keywords:
?-~(35)S-dATP;
Nick translation;
In situ hybridization;
Carbamyl phosphate synthase I(CPS_1)
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
The carbamyl phosphate synthetase I (CPS,) cDNA probe labelled with alpha ~(35)S-dATP was obtained by using modified nick translation technigue. The specific radioactivity of the obtained probe reached 0.9-1.5?10~8cpm/ug DNA. This probe could be satisfactorily used in RNA-DNA in situ hybrioization for detecting the gene expression of CPS_1 in liver tissue sections. The advantages of using ~(35)S instead of ~(32)P and ~3H for labelling DNA probe were discussed.
