THE IMMUNOHISTOCHEMICAL STUDY OF HUMAN OVARIN TISSUE USING MONOCLONAL ANTIBODIES AGAINST PORCINE ZONA PELLUCIDA(ZP-OVA)
- VernacularTitle:猪卵透明带单克隆抗体对人卵巢免疫组织化学研究
- Author:
Quanbin JIANG
;
Yujing GUAN
;
Shujun LIU
;
Jiping WEN
- Publication Type:Journal Article
- Keywords:
ZP-OVA;
Immunohistochemisty;
human ovarian
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Three hybridomas producing monoclonal antibodies (Mab) to zona pellucida of porcine ovarian egg(ZP-OVA) were established(LPDg, LPC4, LPD,).Two Mab(LPD_8 LPC_4) were examined against human ovarian tissue by immunohistochemical technique in vitro. We found that the two Mab had cross-reaction with zona pellucida and ovum cytoplasm and no cross-reaction with membrana granulosa and theca folliculi. It showed that there were affinities of LPD_8 and LPC_4 to antigenic determinamts in the ovum cytoplasm and common antigenic components in zona pellucida and ovum cytoplasm. It suggested that the zona pellucida was derived from oocyte.
