USE OF AMP BUFFER FOR THE FURTHER EXPLORATION ON DETECTION OF LOCALIZATION OF ALKALINE PHOSPHATASE ACTIVITY IN THE LIVER BY THE LEAD CITRATE METHOD
- VernacularTitle:对用AMP缓冲液的柠檬酸铅法显示肝脏ALP酶活性分布的进一步探讨
- Author:
Yuxiu SHI
;
Fujimoto TOYOSHI
;
Ogawa KAZUO
- Publication Type:Journal Article
- Keywords:
AMP(2-amino-2-methyl-l-propanol) buffer;
Alkaline phosphatase;
liver
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to detect the localization of ALPase activity in the liver parenchyma more effectively and make the reaction product finer in EM, AMP (2-amino-2-methyl-1-propanol) buffer has been tried for the exploration on ultrastructural enzyme-histochemistry and biochemical quantitation by the lead citrate method. The microsections were used after fixation with 0.5% glutaraldehyde perfusion.The original Tris-HCl buffer was replaced by 175-350mM AMP buffer and 5mM or 20mM sodium 尾-glycerophosphate were used as substrate. By using all these different concentrations of reaction medium, the ALPase activity in the liver was found in the lateral and sinusoidal surface of hepatocytes as well as in the bile canalicular surface and the surface of mitochondria and lysosome. This suggests that AMP buffer is better and more effective for detection of ALPase activity in liver parenchyma by the lead citrate method.
