THE MORPHOLOGY AND DISTRIBUTION OF THE CHOLINERGIC NEURONAL ELEMENTS IN THE SACRAL AND COCCYGEAL SPINAL CORD OF THE CAT: AN IMMUNOHISTO-CHEMICAL STUDY WITH A MONOCLONAL ANTIBODY TO CHOLINE ACETYL-TRANSFERASE TRANSFERASE
- VernacularTitle:猫骶尾髓胆碱能神经成分的形态和分布——胆碱乙酰化酶单克隆抗体的免疫组织化学方法研究
- Author:
Bingzhi QIN
;
Jishuo LI
- Publication Type:Journal Article
- Keywords:
Cholincrgic neuronal elements;
Choline acetyltransferase;
Sacral and Coccygeal Spinal Cord;
Immurohistochemistry;
Cat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The morphological characteristies and distribution of the choline acetyltransferase (ChAT)-like immunoreactive neuronal elements in the sacral and coccygeal spinal cord of the cat were studied by avidin-biotin combined immunohistochemical technique. The results were as follows:1. The ChAT-positive neuronsIn the ventral horn, ChAT-positive motoneurons were located in the motor columns. These neurons can be divided into three types: the large multipolar neurons, the medium-sized multipolar neurons and the small motoneurons. The color of anti-ChAT reaction product in the large and medium-sized motoneurons varied from light to moderate brown, but the small motoneurons were heavily stained.In the intermediate zone, three groups of ChAT-positive parasympathetic preganglionic neurons were observed in 3 areas: the intermediolateral nucleus (IL) in the lateral part of lamina Ⅶ, the intermediomedial nucleus (IM) dorsal-lateral to the central canal, the intercalated nucleus (IC) looated between IL and IM. The somata of these neurons were round, oval, and fusiform in shape. In the lamina Ⅶ, there were some prominent ChAT medium-sized multipolar neurons named partition cells, scattered among the extended fibers of the parasympathetic preganglionic cells. In the vicinity of the central canal could be observed small CHATpositive cells.In the ventral part of the dorsal commissural nucleus region, a number of ChAT-positive cells were present. They were round, oval and fusiform in shape and small in size (averaging 11.91?m?17.38?m). Occasionally, they were observed in the dorsal part.In addition, some of the ChAT-positive neurons were scattered in laminae Ⅲ-Ⅴ.2. The ChAT-positive fibers and terminals.The ChAT-positive punctate structures were observed throughout the sacral and coccygeal spinal cord resembling terminal branch and terminals. The density of the distribution of the punctate structures was arranged, from high to low, in the following order: the ventral horn, intermediate zone, central gray matter and dorsal commissural nucleus region, and the dorsal horn. In the dorsal horn, many of these punctate structures Were concentrated in two laminae, the major one being lamina Ⅲ and a minor one lamina Ⅰ. But in lamina Ⅱ, they were rare. In the ventral horn such punctate structures were often closely contacted with large and medium-sized ChAT-positive motoneuronal somata and dendrites. Some axonal terminal branch with varicosities closely surrounded a somata and bases of dendrites, and formed contacts with the same somata and its dendrites. In the intermediate zone, there were many transverse fibers associated among the parasympathetic preganglionic nuclei.
