ORIGINS AND ULTRASTRUCTURAL DISTRIBUTION OF SEROTONERGIC FIBERS OF THE SPINAL DORSAL HORN IN THE RAT——A STUDY WITH COMBINED METHOD OF HRP AND IMMUNOCYTOCHEMISTRY AND IMMUNOELECTRON MICROSCOPIC OBSERVATION
- VernacularTitle:脊髓背角5-羟色胺纤维的来源和超微结构分布——HRP与免疫细胞化学结合法研究和免疫电镜观察
- Author:
Qingying LIU
;
Changgeng ZHU
;
Hantao LIU
;
Jinhuo LU
- Publication Type:Journal Article
- Keywords:
Serotonin;
Spinal dorsal horn;
Synapse;
Immunocytochemistry;
Immunoelectron microscopy
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Origin and ultrastructural characteristics of serotonergic fibers of the spinal dorsal horn in the rat have been confirmed by means of a combined method of HRP and immunocytochemistry and immunoelectron microscopic observation. The results showed that serotonergic axonal terminals in the spinal dorsal horn come mainly from nucleus raphe magnus and the ventral part of the reticular formation of medulla oblongata. Serotonin immunoreactive positive structures of the spinal dorsal horn have been found in lamina Ⅰ (marginal zone) and lamina Ⅱ (substantia gelatinosa) as fine myelinated and unmylinated fibers. There were mainly axo-axonic synapses between the labeled and nonlabeled terminals. The labeled terminals were presynaptic or postsynaptic element. Axo-dendritic synapses were rarely found. The non-synaptic releasing figures have not been found. Based on the ultrastructural characteristics the authors suggest that in performing analgesia role the serotonergic system in the spinal dorsal horn might influence directly or indirectly the excitability of interneurons and inhibit directly the nerve impulses of primary afferents by means of synaptic connections instead of non-synaptic releasing manner.
