STUDY OF PROLIFERATIVE CYCLE OF RAT HEPATOCYTES AFTER PARTIAL HEPATECTOMY USING FLOW CYTOMETRY
- VernacularTitle:应用流式细胞计对大鼠肝大部切除后肝细胞增殖周期动态的研究
- Author:
Jihong ZHU
;
Lingzhong CHENG
;
Cuiping ZHONG
;
Yundi GU
- Publication Type:Journal Article
- Keywords:
Cell cycle;
Liver regeneration;
Flow cytometry;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Thirty-six male adult rats were divided into 12 groups. The rats, except the control group, were partially (68％) hepatectomized and then killed at intervals between 12-120h after operation. Isolated hepatocytes were prepared and flow cytometry was used to study the proliferative cycle. Mitotic index and binuclear cell count have been performed in liver sections and smears of isolated hepatocytes separately. Tetraploid cells acounted for 76% of all hepatocytes in normal rats and they also constituted the main proliferative population in regenerating liver. During 12-20h after operation, some of the tetraploid cells that remained in G_2 phase entered into mitosis. At 24h after operation, peaks of S phase in tetraploid cells and of octoploid cells occurred. At 36h after operation, mitotic index reached a maxium value and thereafter the percentage of binuclear cells were reduced rapidly. At 48-72h after operation, second peak of DNA synthesis occurred, but showed wide individual variations in time and cell proportion.
