CYTOCHEMICAL LOCALIZATION OF 3',5'-CYCLIC NUCLEOTIDE PHOSPHODIESTERASE ACTIVITY WITH ELECTRON MICROSCOPY IN RAT THYROID
- VernacularTitle:大白鼠甲状腺细胞膜系统3′,5′-环核苷酸磷酸二酯酶活性的电镜细胞化学定位
- Author:
Hao WANG
;
Rongzhen LIU
;
Guiwen RUAN
- Publication Type:Journal Article
- Keywords:
Thyroid;
Localization of 3′,5′-cyclic nucleotide Phosphodiesterase;
Electron microscopy cytochemistry;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Localization of 3', 5'-cyclic nucleotide phosphodiesterase activity in thyroid follicular cells and endothelium was examined by cytochemical methods for electron microscopy. In thyroid follicular cells reaction product deposition associated with 3', 5'-cyclic nucleotide phosphodiesterase activity was localized at the plasma membrane. In these cells, the enzyme activity appeared mostly localized on the apical and lateral plasma membrane, and also appeared on the outer surface of nuclear envelope, endoplasmic reticulum and Golgi apparatus. However, when cAMP and snake venom were omitted simultaneously, no enzyme activity was observed. 3', 5'-cyclic nucleotide phosphodiesterase activity also appeared on the luminal surface of endothelium of small blood vessels.
