A STUDY ON MALIGNANT TRANSFORMATION OF C_3H/10T 1/2 CELLS INDUCED BY CHEMICAL CARCINOGEN (BP)
- VernacularTitle:化学致癌剂3-4苯并吡诱发C_3H/10T 1/2细胞恶性转化的研究
- Author:
Changqing PIAO
;
Shuqing GE
- Publication Type:Journal Article
- Keywords:
C_3H/10T-(1/2) cell line;
3, 4 Benzopyren (BP);
Malignant transformation;
Transformed foci
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
C_3H/10T 1/2 cell line was used for the study of malignant transformation induced by chemical carcinogen-Benzopyren (BP). The results showed that the transformed foci developed in 7—9 weeks after being attacked by BP. The transformed cells separated from the foci lost their ability of post-confluence inhibition of division, and could grow into colony in soft agar medium. The transformed cells have produced sarcoma in situ after inoculation into the immunosuppressed animals. The above results suggest that C_3H/10T(1/2) cell line is very useful for the study of malignant transformation of cells in vitro induced by carcinogens.In chromosome analysis, the changes in number and structure of chromosome in the transformed cells were found. The abnormality of chromosome proved the close relationships between the malignant transformation of cells and the DNA damage induced by chemical carcinogens
