A HISTOCHEMICAL OBSERVATION ON ISOLATED BONE CELLS FROM CALVARIUM OF RAT EMBRYOS CULTURED IN VITRO
- VernacularTitle:胚胎大鼠颅盖骨分离细胞早期体外培养的组织化学观察
- Author:
Ronghui XU
;
Yaping ZHU
;
Benfu CHAI
- Publication Type:Journal Article
- Keywords:
Histochemistry;
Bone cells;
Cell culture;
Rat embryos
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
An experimental study was carried out on the isolated bone cells, from the embryonic calvarium of Sprague-Dawley rats which were taken out in vitro culture in the later period of gestation. Observation of the early stage of culture was carried out with phase contrast microscope, H. E. staining and AcP and AlP technique. It was found that both the osteogenic cells of the cambium layer of the periosteum and the undifferentiated cells from the bone marrow could develop into large squamous-shaped cells. These cells possessed many processes connected with the adjacent cells and stained light red color with Sirius Red but revealed no birefringency under polarized microscope. This indicates that these cells are precursors of collagen formation. The negative reaction with the alkaline phosphatase staining pointed out that these cells still belong to the osteogenic cells.
