CIRCADIAN VARIATIONS OF PITUITARY ?-ENDORPHIN-LIKE IMMUNOREACTIVITY AND ITS RELATIONSHIP TO THE PAIN THRESHOLD IN RATS
- VernacularTitle:大鼠垂体?-内啡肽样免疫反应性的昼夜变化节律及其与痛阈的关系
- Author:
Shumin ZHAO
;
Jinglan WU
- Publication Type:Journal Article
- Keywords:
?-endorphin;
Pain threshold;
Circadian variation;
Pituitary;
Immunohistochemistry;
Radioimmunoassay;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Immunohistochemical method, immuno-electron microscopic technique and radioimmunoassay (RIA) were used for the demonstration of ?-endorphin-like immunoreactivity (?-EP-IR) and of the circadian variation of the ?-EP-IR level in rat pituitary. ?-ER-IR was located in all cells of the pituitary intermediate lobe. A few ?-ER-IR-positive cells were scattered in the anterior lobe, and no positive immunoreactivity was found in the posterior lobe. The controls were negative. Under electron microscope, ?-ER-IR-positive dense particles were mostly located in the secretion granules, and a few of them were scattered freely in the cytoplasm of the intermediate lobe cells. Both pituitary ?-EP-IR concentration and the basal pain threshold in rat showed circadian variations in the experiment done at 4-hr intervals over a 24-hr period (P
