EFFECT OF EXOGENOUS cAMP ON HUMAN STOMACH GLANDULAR CARCINOMA SGC-7901 CELLS
- VernacularTitle:外源性3′,5′,环腺苷酸对人胃腺癌细胞系的影响
- Author:
Guizhi LU
;
Yan GAO
;
Yanchuan HUANG
;
Ling PENG
;
Kunren WANG
- Publication Type:Journal Article
- Keywords:
3',5' adenosine monophosphate (cAMP);
cAMP-phosphodiesterase(cAMP-PDEase), Mg~(++)-ATPase;
Human stomach glandular carcinoma SGC-7901 cell line
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The experiment was carried out on cultured human stomach glandular carcinoma (SGC-7901 cell line). We previously reported the cytochemical changes of (Na~+-K~+)-ATPase activity and the changes of microvilli. We here studied the relationship among the cAMP, cAMP-PDEase, Mg~(++)-ATPase activity, the cell growth and differentiation. Cells were exposed to Immol/L cAMP together with 1 mmol/L theophylline. We showed cAMP specific fluorescence by immunocytochemical method, Mg~(++)-ATPase and cAMP-PDEase activity by cytochemical methods. We found that cAMP with theophylline not only inhibited the growth of these SGC-7901 cells as shown by our previous result, but also decreased the activity of Mg~(++)-ATPase and 3', 5'-cyclic monophosphate phosphodiesterase. The result indicates that exogenous cAMP can inhibit 3', 5'-cAMP-PDEase and Mg~(++)-ATPase activity and increase cAMP level in cell, and cAMP regulated the growth and differentiation of SGC-7901 cell line
