THE ESTABLISHMENT OF HGPRT MUTANT FROM HUMAN STOMACH GLANDULAR CARCINOMA (BGC 823)
- VernacularTitle:人胃癌次黄嘌呤-鸟便嘌呤磷酸核糖转移酶缺陷株的建立
- Author:
Jingsheng TIAN
;
Aimin WANG
;
Chunjing WU
;
Guifang JIN
- Publication Type:Journal Article
- Keywords:
Human cell line;
HGPRT mutant;
Human stomach glandular carcinoma cell
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The wild type cells of human stomach glandular carcinoma, cell line BGC 823, were treated firstly by a chemical carcinogen (MNNG) for the induction of mutagenesis, then the cells were selected in medium with gradually increasing amount of 8-AG (8-azaguanine) from 1-20?g/ml. It was found that the mutant cells could grow vigorously in the medium containing 20?g/ml of 8-AG but not in HAT medium. The HGPRT assay showed an obvious quantitative difference between the wild type and HGPRT mutant cells of BGC 823.
