IMMUNOCYTOCHEMICAL LOCALIZATION OF EPIDERMAL KERATIN IN THE RAT HEPATOMA CELL LINES FSK 7901, 7902 AND THEIR SOLID TUMORS
- VernacularTitle:大白鼠肝癌细胞系FSK7901, FSK 7902及其实体瘤的表皮角蛋白免疫细胞化学定位
- Author:
Qin SU
;
Minzu GONG
;
Yanfang LIU
- Publication Type:Journal Article
- Keywords:
Rat hepatoma cell lines FSK 7901 and 7902;
Solid tumors;
Epidermal keratin;
Immunocytochemical localization
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
We prepared bovine epidermal keratin (EK) antibody, which can be used as a specific marker of cholangiocarcinoma(CC) cells to be distinguished from hepatocellular carcinoma (HCC) cells. The intrahepatically implanted solid tumors of FSK 7901 and FSK 7902 have the histological structures of CC and HCC, respectively. Using immunofluorescence and PAP techniques, we demonstrated that, in the solid tumors, FSK 7901 cells are definitely EK-positive, while most of the cells of FSK 7902 are EK-negative. Therefore, we considered FSK 7901 and FSK 7902 as CC and HCC, respectively. In the solid tumors of FSK 7902, few cells which are EK-positive may be those in which there are Mallory bodies or the structures are forming.
