IN VITRO FERTILIZATION AND CULTURE OF HUMAN OVA
- VernacularTitle:人卵的人工受精和体外培养
- Author:
Bin LIU
;
Ying LI
;
Yan CHANG
- Publication Type:Journal Article
- Keywords:
Fertilization in vitro;
Culture in vitro;
Embryo transfer;
Human ova
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In the experiments we used T_6 medium prepared by ourselves and mixed with diferent concentrations of human serum albumin or mother's serum, from which we prepared follicular flushing medium, insemination medium and growth medium. Clomiphene citrate and HCG were used for inducing superovulation. The semen was obtained through masturbation and artificially capacitated. A mature ovum and 2-3 drops of capacitated semen were incubated together in insemination medium in the culture box containing 5% CO_2 in air atmosphere at 37℃. The ovum was aspirated and transferred into the growth medium and incubated for 16-17 hours. By this time the ovum was already fertilized with the appearance of 2 pronuclei. About 30 hrs, it normally developed into two-cell stage, 40-44 hrs, it cleavaged into 4-cell stage. After 72 hrs, the fertilized ovum grew into morula. Up to June, 1986 twentythree ova in vitro fertilized and cultured were transfered into 14 mothers, uteri at the four-cell stage. The details of fertilization and the early embryo development in vitro were described.
