MALIGNANT TRANSFORMATION OF MIXED CULTURE CELLS OF LUNG AND LIVER OF NEW BORN MOUSE INDUCED BY~3H-TdR AND ITS CHROMOSOME ANALYSIS
- VernacularTitle:~3HTdR诱导乳鼠肺肝混合培养细胞的恶性转化及其染色体分析
- Author:
Changqing PIAO
;
Yuan YANG
;
Shuqing GE
- Publication Type:Journal Article
- Keywords:
Culture cells;
Malignant transformation;
Chromosome analysis;
Lung;
Liver
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this experiment, primary mixed culture cells of lung and liver derived from new born mouse was made use of target cells, 0.1 ?Ci 3~H-TdR per milliliter medium was added in the culture in order to induce malignant transformation of the cells in the culture. Results of the experiment was that the cells effected by 3~H-TdR had a unlimited growing and formed sarcoma after being inoculated into new born mice immunosuppressed with ATS. It suggested that they had became malignant transformation cells. Results of analysis of chromosome aberrations of the transformed cells, the long arm chromosome was observed in 5% of cells, the metacentric chromosome in 7% of cells, the acentric fregment in 8% of cells. It shows that DNA damage of the cells induced by 3~H-TdR causes their chromosome aberrations and, futhermore, development of malignant cells. The fact that unstable aberrations was Still in sight in the malignant transformation cells suggested that there have been a bit of 3~HTdR left in these cells which kept damaging DNA of the cells.
