ULTRASTRUCTURAL LOCALIZATION OF MONOAMINE OXIDASE AND THAMINE PYROPHOSPHATASE ACTIVITY IN THE CELLS OF SYMPATHO-ADRENAL MEDULLAR SYSTEM IN THE GUINEA PIG
- VernacularTitle:单胺氧化酶和硫胺素焦磷酸酶在豚鼠交感——肾上腺髓质系统细胞超微结构内的定位
- Author:
Guang YANG
;
Zhaote LI
- Publication Type:Journal Article
- Keywords:
Sympathetic ganglion;
Adrenal medulla;
Small intensely fluorescent cell;
Cytochemistry;
Monoamine oxidase;
Thiamine pyraphosphatase;
Guineapig
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Monoamine oxidase and thiamine pyrophosphatase activities were demonstrated ultracytochemically in the celiac ganglion and adrenal medulla of the guinea pig by Shannon's and Novikoff's method respectively. Monoamine oxidase activity was found frequently on the nuclear evelope, and ocassionally in mitochondrial outer compartment and cell membrane of the small intensely fluorescent (SIF) cells of the ganglion. Similar Iocalizations were also observed in chromaffin cells of the adrenal medulla. In pricipal neurons of the ganglion, a large amount of products of the monoamine oxidase reaction were found on the endoplasmic reticulium in addition to the nuclear envelope and mitochondrial membranes. Abundant thiaminepyrophosphatase activity was seen at the tran-face of the Golgi complex in the pricipal neurons, in contrast to which, both SIF cells and chromaffin cells exhibited little thiamine pyrophosphatase activity. The results suggested that catecholamine metabolism and the mode of functional activity in the SIF cells were different from those in the pricipal neurons but similar to those in the chromaffin cells of adrenal medulla.
