LIGHT AND ELECTRON MICROSCOPY OBSERVATION ON ACTIVITIES AND LOCALIZATION OF 5'-NUCLEOTIDASE AND THIAMINE PYROPHOSPHATASE IN THE HUMAN PROSTATE HYPERPLASIA
- VernacularTitle:前列腺良性增生的5′-核苷酸酶和硫胺素焦磷酸酶定位及其活性的观察
- Author:
Lianrong SHI
;
Yi ZHANG
;
Tianmin PAN
- Publication Type:Journal Article
- Keywords:
5'-Nucleotidase;
Lysosome;
Golgi vacuole and vesicle;
Golgi saccule;
Thiamine pyrophosphatase;
Actinomyces globispores;
Estrogen
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The epithelial cells of human prostatic hyperplasia had been studied cytochemically for 5'-nucleotidase and thiamine pyrophosphatase activity with light and electron microscopy. The 5'-nucleotidase (5'-Nase) activities were present in the epithelial cells at lysosomes, Golgi vacuoles, vesicles, secretory vacuoles and free surface of plasmic membrane, the secretion material within the acinar lumina of the prostate gland also showed 5'-nucleotidase reaction product. The activity of 5'-nucleotidase has not markedly changed by the actinomyces globispores treatment, but slightly decreased after estrogen treatment. It was no enzyme reaction after treated by testectomy.Activities of thiamine pyrophosphatase (TPPase) were restricted to the Golgi area, and there were heavy deposits of reaction products in concave face of Golgi saccule. The activities of TPPase were not markedly changed by estrogen and actinomyces globispores treatments but none of enzyme activities could be seen after testectomy.
