THE OBSERVATION ON THE BEATING AND GROWTH CHARACTERISTICS OF THE PRIMARY MYOCARDIAL TISSUE CULTURE OF HUMAN FETUS
- VernacularTitle:人胎儿心肌组织原代培养搏动及其生长特点的观察
- Author:
Peisun YANG
;
Jin GAO
- Publication Type:Journal Article
- Keywords:
Human;
Fetus;
Myocardium;
Tissue culture;
Spontaneous beating;
Growth characteristic
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Myocardial tissue masses were isolated from two human fetus of 10 and 16 week's gestation respectively and they were cultured under the conditions for cultivating the myocardial cells of the rodents. Spontaneous beating occured. In one case the frequency of beating was 21～27 beats/min, and the beating lasted for 30 days until the culture was terminated artifitially. In another case a cell mass continued to beat as long as 75 days with a frequency of 200～290 beats/min, and then stopped spontaneously. The differences in thickness and shapes of the tissue masses would influence the modes of growth after culturing. Thicker tissue mass grew more slowly and the fibroblasts and myocardial cells grew and spreaded out radially, or in band form. In tissue mass with a thin border the marginal myocardial cells bulged out in globular shape in the early stage and then separated from the cell mass and continued to grow. In thin layered tissue mass, because of the rapid overgrowth of the surrounding fibroblasts, the myocardial tissue would grow and overlap them and increased steadily in thickness. This procedure is simple and relatively stable, and possible to retain the long-lasting beating of the myocardial tissus, but it is difficult to obtain a pure myocardial cell culture for cell counts and other quantitative studies.
