SOME MODIFICATIONS OF IN VITRO FERTILIZATION TECHNIQUE FOR HUMAN SPERMATOZOAL PENETRATION INTO HAMSTER OOCYTES
- VernacularTitle:人类精子与金黄地鼠卵体外受精技术的某些改良
- Author:
Jingbo ZHANG
;
Yuzhou YANG
;
Ju YUAN
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
We describe a modified technique for the in vitro study of human spermatozoal penetration into hamster oocytes which is easier to master and more simple to perform and needs no complicated facilities.It comprises the following aspects:1 The hamster oviduct is stained with 1％ neutral red agar slics, so that the opening of the oviduct is easy to find and easy to be inserted with needle for flushing eggs.2. A special small tube,3 cm long with a diameter of 0.5 cm is used for fertilization instead of plastic dish with paraffin oil covering the media.3. Ordinary incubator is used rather than CO_2 incubator. With these procedures, the sperm penetration rates we got were 75％, 25％, 40％, 80％ and 46％ respectively in 5 experiments. It could meet the needs to evaluate the fertilizing capacity of human spermatozoa.
