AN ULTRASTRUCTURAL STUDY OF SMALL INTENSELY FLUORESCENT (SIF) CELLS IN CELIAC GANGLION OF GUINEA PIG, WITH SPECIAL REFERENCE TO THE CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE
- VernacularTitle:豚鼠腹腔神经节小强荧光(SIF)细胞的超微结构及细胞化学研究
- Author:
Guang YANG
;
Zhaote LI
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The celiac ganglia from 9 mature guinea pigs of both sexes were fixed in a mixture of 2％ glutaraldehyde and 2.5％ depolymerized paraformaldehyde. The SIF cells in cryostat sections were discerned out through their eatecholamine fluorescence induced by the fixative. These cells were processed through routine procedures for electron microscopy after or without incubation for acid phosphatase (AcP). The ultrastructure and AcP activity of these cells were observed under an H-600 electron microscope.The SIF cells were found to be present in clusters adjecent to fenestrated capillaries. The exocytosis of vesicular granules of SIF cells were seen on the surface of their cell membrane facing the capillaries. In addition, some dark SIF cells which were more osmiophilic and rich in mitochondria were discovered in this ganglion. These ultrastructural features of the SIF cells indicate that they may perform functions of an endocrine and/or paracrine nature.After one. hour incubation in a Gomori-typed medium at 37℃, some granules. besides the typical lysosomes display the AcP activity. Although the nature of the AcP-positive granules hasn't been elucidated a speculation is laid on that the presence of the enzyme on these granules may play a role of regulation in replacing the intragranular contents.
