THE OBSERVATION OF THE CHANGED PROCESS OF SPECIFIC SURFACE STRUCTURE OF HUMAN NASOPHARYNGEAL CARCINOMA CELL LINE(CNE)IN VARIOUS TIMES OF CULTIVATION BY SCA NNING ELECTRON MICROSCOPE
- VernacularTitle:在扫描电镜下观察培养不同时间的人鼻咽癌细胞株(CNE)表面特殊结构变化过程
- Author:
Jin GAO
;
Jian YANG
;
Baogui LI
;
Kexun XUE
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
CNE cells were cultured on cover glasses placed in the culture flasks, and were examined under scanning electron microscope 6,24, 48,72, and 96 hours after cultivation. The results were as following: The surface structures of tumor cells changed in different intervals of cultivation. Evenly distributed zeiotic blebs were found on the surface of tumor cell after 24 hours of cultivation and most of these blebs were seen at marginal areas of tumor cells after 2 days of cultivation, and finally disappeared after 3 days of cultivation. In addition to the blebs some microvilli which had increased since the second day of cultivation were seen over the margin of tumor cells. On the third day of cultivation a large number of microvilli were seen distributed over the entire surface of tumor cells. Besides microvilli and zeiotie blebs, lamellipodia and ruffles were also observed after 24 hours, but vanished after 48 hours of cultivation. The filopodia were seen over the whole process of cultivation. They usually divided into 2、3 branches and the expanded ends looked like suckers. All these data indicated that the surface structure of tumor cells were variable in different periods of cultivation.
