STUDIES OF THE GOSSYPOL EFFECTS ON THE CHROMOSOME ABERRATION AND SISTER CHROMATID EXCHANGE (SCE) IN MOUSE SPERMATOGONIA IN VIVO
- VernacularTitle:棉酚对小鼠活体精原细胞染色体畸变及姐妹染色单体交换(SCE)率影响的研究
- Author:
Yuzhou YANG
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Substitution of thymidine with 5-Bromo-deoxyuridine (BUdR) was performed in vivo in mice for the detection of gossypol effects on the chromosome aberration and sister chromatid exchange (SCE) in spermatogonia. The male Kun-ming mice received a daily dose of 4mg/kg of gossypol for two weeks, followed by a single injec- tion intraperitoneally of BUdR adsorbed on activated charcoal. Metaphases from spermatogonia were prepared for observation 54 hours after the injection.The results demonstrated that the mean frequencies of chromosome aberration and SCE in spermatogonia of gossypol treated mice did not differ significantly from that of the controls. Whereas, the incidences of SCE in mice had been exposed to known mutagen mitomycin C which used as positive controls were markedly increased. This data provide evidence of more sensitive indices for chromosome damage to support the notion that gossypol was not a mutagenic agent.The method of in vivo analysis of SCE in spermatogonia and its application and significance in the detection of genetic damage of contraceptive agents was discussed.
