THE METABOLIC CHANGES OF ADRENALINE—INDUCED MYOCARDLAL LESIONS. A REPORT OF HISTOCHEMICAL STUDY
- VernacularTitle:肾上腺素致病性心肌病变的代谢变化——组织化学的观察报告
- Author:
Chiakuei MU
;
Chingchuan CHU
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A total of 26 adult rabbits were used in this study. Six were used as control andthe remaining 20 rabbits were injected with adrenaline to produce focal cardiac lesions.These animals were sacrificed alternatively either by medulla shock or calcium arrest after1, 3, 5, 7, 10, 14 and 21 days of experiment. The left ventricle was excised and sectioned.To the unfixed frozen sections, Chevremont and Frederic method for SH-group were used(Mecurric chloride or Mecurric acetate block were used as control), also Sudan blackB were used for staining fat. To paraffin sections, H-E and McManus PAS method(saliva-digestion as control) were stained to demonstrate the general structure and theglycogen. The following points of conclusion were drawn: 1) The adrenaline induced necrotic myocardial fibers were saliva-resisted PASpositive. 2) There were many degenerative fibers at the sides of the necrotic fibers orarround the lesions. These fibers showed fatty change, deposition of abnormal glycogenand deeper stain for SH-reaction. These fibers might regain to normal or progress tonecrotic. 3) The fatty change and the deposition of abnormal glycogen first appeared at thefirst day of the experiment. On the recovery of the muscle fibers, the abnormal glycogenregained earlier to normal than that of the fatty change. From this, the authersbelieved: The fatty change might be caused by the abnormal metabolism of the glycogen.This again proved the authorized work of pathologists: Fat burns in the flame of carbo-hydrate. 4) The relationship between the increase of SH-group and both the fatty changeand the abnormal glycogen deposition was discussed. 5) Of the fragmented cardiac fibers, the glycogen granules disappeared before itsnecrosis, and the fatty granules were removed later by the macrophages.
