HISTOLOGICAL AND HISTOCHEMICAL CHANGES OF RABBIT PARATHYROID DURING THE HEALING OF EXPERIMENTAL BONE FRACTURE
- VernacularTitle:实验性家兔骨折愈合过程中甲状旁腺的组织学和组织化学的变化
- Author:
Guangfang SUN
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Ninety-five adult male rabbits were used in this experiment. Except 18 controlanimals, the rest, with a segment of radius sawed off under surgical operation, weredivided into different experimental groups. They were sacrificed at different time inter-vals and their parathyroid glands were dissected out immediately and processed accor-ding routine histological and histochemical methods. Three days after bone-fracture the parenchyma cells increased in size and in numberof vacuoles in the cytoplasm. The intensity of the ninhydrin-Schiff reaction and reactionof the alpha-acylamino carboxyl groups of proteins also increased. These changes in-tensified on the 7th day and did not recover to normal on the 35th day after opera-tion. On the 7th day the parenchyma cells also showed an increase of ribonucleic acid, adecrease in glycogen content, a rise in the activity of the enzymes: nonspecific esterase,acid phosphatase, and succinic dehydrogenase; and the capillary endothelium showed anelevation of alkaline phosphatase. On the 17th day the activity of esterase and succinic dehydrogenase subsided to nor-mal and on the 21st day the glycogen content and alkaline phosphatase activity returnedto normal. The above changes are correlated with the different stages of the bone healing pro-cess and their significance in parathyroid gland activity is discussed.
