ELECTRON MICROGRAPHY SHOWING THE FORMATION OF MITOCHONDRIA AND GRANULATED ENDOPLASMIC RETICULUM
- VernacularTitle:关于线粒体及动质来源的一些电子显微镜观察
- Author:
Tsokan CHANG
;
Hweiyun MA
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The apical ectodermal thickening of the limb buds of mouse embryos, 11-day vaginalplug age, and epithelial cells of the villi of small intestine, 19-day mouse fetus, wereused for this observation. The limb bud ectoderm was fixed in 1% osmic acid andstained in 1% Pb(OH)_3, while the small intestine was fixed in 1.2% potassium per-magnate. There were transitional stages from minute dense vesicles of 0.01--0.07? with afew vague striations, gradually growing up into vesicles of 0.07--0.10? with definitemitochondrial cristae, and finally into organelles that could be definitely identified assmall mitochondria of 0.13?0.20? in size, They were neither fragments of degenera-ting mitochondria nor lysosomes, since their structures became more and more similar tothat of the mitochondria with their increase in size. These minute dense vesicles werefound among clusters of ribosomes, at first vaguely outlined and then with definite mem-branes and dense substance. The significance of ribosomes in relation to synthesis of themembranous protein and the matrix of the mitochondria was discussed. In the course of the formation of granulated endoplasmic reticulum, diffuse cyto-plasmic ribosomes at first arranged themselves into circular or tubular clusters. Theseclusters then transformed themselves into membrane bounded tubules and vesicles. Byfurther fusion and extension, definite granulated endoplasmic reticula were formed.Close relationship between mitochondrium, granulated endoplasmic reticulum and Golgibody has been observed but there showed no evidence of developmental significance.The granulated endoplasmic reticulum was thus considered to be formed by ribosomes.
