The role of PD-ECGF and DPI in hepatic metastasis of patients with colorectal carcinoma
- VernacularTitle:肝脏血流灌注指数和血小板衍生内皮细胞生长因子在结直肠癌肝转移中的作用研究
- Author:
Hao GUO
;
Zhixiang ZHANG
;
Liwei ZHU
- Publication Type:Journal Article
- Keywords:
colorectal neoplasms;
neoplasm metastasis;
Doppler perfusion index;
platelet derived-endothelial cell growth factor
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in Doppler perfusion index(DPI)of the liver and the platelet derived-endothelial cell growth factor(PD-ECGF)in patients with liver metastasis from colorectal carcinoma by determination DPI and PD-ECGF mRNA and protein expression levels.Methods One hundred patients were enrolled,including 50colorectal carcinoma patients(22of whom with liver metastasis and 28patients without liver metastasis)and 50healthy subjects as control.For all the objects involved,hepatic perfusion was documented with color Doppler flow imaging,and the DPI(hepatic arterial/total liver blood supply)was computed.The expression levels of protein and mRNA of PD-ECGF in 50specimens of carcinoma and adjacent tissues of colorectal carcinoma were assayed by immunohistochemistry and real-time fluorescence quantitative PCR methods in patients with colorectal carcinoma.Results According to pathology and imaging results,the DPI of colorectal carcinoma patients with liver metastasis was significantly higher than that in healthy control group and non-liver metastasis group(P0.05).According to the results of immunohistochemistry and real-time fluorescence quantitative PCR,the expression levels of PD-ECGF protein and mRNA were significantly higher in colorectal carcinoma than that in adjacent tissues(P
