Role of UCP2 Expression after Hepatic Warm Ischemia-Reperfusion in the Rat.
- Author:
Mizuki NINOMIYA
1
;
Ken SHIRABE
;
Mitsuo SHIMADA
;
Takahiro TERASHI
;
Yoshihiko MAEHARA
Author Information
1. Department of Surgery and Science, Kyushu University, Fukuoka Graduate School of Medical Sciences, Fukuoka, Japan. nino-m@surg2.med.kyushu-u.ac.jp
- Publication Type:Original Article
- Keywords:
Oxidative stress;
Ischemia-reperfusion;
Uncoupling protein;
Liver;
Surgery
- MeSH:
Animals;
Hepatocytes;
Immunohistochemistry;
Ion Channels;
Ischemia;
Liver;
Malondialdehyde;
Mitochondrial Proteins;
Oxidative Stress;
Rats;
Rats, Wistar;
Reperfusion
- From:Gut and Liver
2011;5(4):486-492
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The role of uncoupling protein-2 (UCP2) in the liver is currently unclear. Emerging evidence suggests a relationship between UCP2 and oxidative stress. In the present study, we tested the hypothesis that UCP2 expression in the liver might change during warm ischemia-reperfusion (I/R) according to oxidative stress. METHODS: Wistar rats were subjected to 40 (short ischemia) or 90 (long ischemia) minutes of partial lobar ischemia followed by 4 hours of reperfusion. UCP2 expression in the ischemic and nonischemic lobes was assessed using reverse transcription-polymerase chain reaction and immunohistochemistry. Malondialdehyde concentrations in the liver tissue were also compared. RESULTS: Malondialdehyde concentrations in the ischemic lobes were significantly higher in the long ischemia group. In the ischemic lobes of the short ischemia group, UCP2 protein expression was induced in hepatocytes, which did not express the protein prior to treatment, and the expression levels were higher than in the long ischemia group. The intralobular distribution of UCP2 seemed to correlate inversely with that of the necrotic area. UCP2 expression was observed, even in nonischemic lobes with similar intralobular heterogeneity. CONCLUSIONS: UCP2 was induced in hepatocytes after warm I/R. Although the primitive role of UCP2 expression may be cytoprotective in nature, its actual protective effect in hepatic I/R may be minimal
