Studies on HPLC fingerprint of Herba Sedum aizoon L
- VernacularTitle:养心草药材HPLC指纹图谱研究
- Author:
Zhucan LIN
;
Dan LIN
;
Fei XU
;
Suhua GUO
- Publication Type:Journal Article
- Keywords:
Sedum aizoon L;
pickingtion;
Fingerprint;
HPLC
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The fingerprint chromatograms were established for quality evaluation of Sedum aizoon L.collected from different habitats by HPLC.Methods:The analysis was performed on a Diamonsil C18 column(4.6mm? 250mm,5?m)with acetonitrile-water(acidified to 0.5%with phosphoric acid)as mobile phase in a gradient mode at a flow rate of 1.0 mL/min,and at a column temperature of 25℃.The detection of wavelength was at 254 nm.Results:2lpeaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 3%in the precision and repeated test.The similarity of l0 batches of samples were no less than 0.9.Conclusion:The method was reliable and can be helpful on the quality control of Sedum aizoon L.
