Heat Shock Proteins and Autophagy in Rats with Cerulein-Induced Acute Pancreatitis.
- Author:
Jin Nam KIM
1
;
Hong Sik LEE
;
Soo Hyung RYU
;
You Sun KIM
;
Jeong Seop MOON
;
Chang Duck KIM
;
In Youb CHANG
;
Sang Pill YOON
Author Information
1. Division of Gastroenterology, Department of Internal Medicine, Seoul Paik Hospital, Inje University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Acute pancreatitis;
Autophagy;
Heat shock protein
- MeSH:
Amylases;
Animals;
Autophagy;
Blotting, Western;
Caerulein;
Cathepsin B;
Heat-Shock Proteins;
Hot Temperature;
Injections, Intraperitoneal;
Lysosomes;
Microscopy, Electron;
Pancreatitis;
Rats;
Trypsinogen;
Water
- From:Gut and Liver
2011;5(4):513-520
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of cerulein-induced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy. METHODS: Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 microg/kg) or saline, respectively. All of the rats were sacrificed at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II. RESULTS: WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy findings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy. CONCLUSIONS: HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy.
