Protective effects of ginsenosides on oxidative damage in mouse spermatogonial cells
- VernacularTitle:人参皂苷保护小鼠精原细胞氧化损伤的研究
- Author:
Dalei ZHANG
;
Bei YANG
;
Lei WU
;
Ting ZOU
- Publication Type:Journal Article
- Keywords:
ginsenosides;
spermatogonial cells;
malon-dialdehyde;
superoxide dismutase;
glutathione;
reactive oxygen species;
oxidative damage
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effects of ginsenosides ( GS) on reactive oxygen species-induced oxidative damage in mouse spermatogonial cells. Meth-ods Mouse spermatogonial cell oxidative stress model was established and the attenuating effects of ginsen-osides on germ cell oxidative damage were evaluated by determination of cell viability,malondialdehyde( MDA) formation,superoxide dismutase ( SOD) activity and glutathione ( GSH) level. Results The exposure to hypoxanthine/xanthine oxidase ( HX/XO) induced an elevation in MDA,while a decrease in germ cell viability,SOD activity and GSH level. However,supplementation with GS ( 10 mg?L -1) restored HX/XO-induced decrease in cell viability,SOD activity and GSH level and HX/XO-induced increase in MDA formation. Conclusion GS may exert antioxidant activity to attenuate reactive oxygen species-induced oxidative damage in mouse spermatogonial cells.
