Curcumins upregulate expression of HO-1 via inducing Nrf-2 in SH-SY5Y cells
- VernacularTitle:姜黄素通过诱导Nrf-2上调SH-SY5Y细胞中HO-1的表达
- Author:
Wenke YIN
;
Xiaodong SHI
;
Xiong ZHANG
;
Yu LI
- Publication Type:Journal Article
- Keywords:
heme oxygenase-1;
nuclear factor erythroid 2-related factor 2;
curcumin;
cytoprotection;
SHSY5Y cell;
induction;
neurodegenerative disease
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Curcumin on the expression of HO-1 and Nrf-2,and explore the machanism of neuroprotection of Curcumin. Methods SH-SY5Y cells were treated with Curcumin at 0,1. 25, 5. 0,20. 0 ?mol?L -1,or with Curcumin at 5. 0?mol? L -1 for 12,24,48 h for the time course assay. RT-PCR and Western blot assays were carried out to detect mRNA and protein expression of Nrf-2 and HO-1. Nrf-2 siRNA was used to dectect the expression of HO-1 with the absence of Nrf-2. Results RT-PCR and Westernblot results indicated that the mRNA and protein expression of HO-1 and Nrf-2 all increased in a dose-and time-dependent manner after Curcumin treatment in the SH-SY5Y cells ( P
