Method studies of two-dimensional polyacrylamide gel electrophoresis of serum
- VernacularTitle:血清样本双向电泳实验方法学研究
- Author:
Xin ZHAO
;
Xiaoping PU
;
Weizhong XIAO
- Publication Type:Journal Article
- Keywords:
proteomics;
serum;
2-DE;
albumin;
sample pretreatment;
silver nitrate staining
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish an ideal two-dimensional polyacrylamide gel electrophoresis( 2-DE) method for serum proteomic research. Methods The 2-DE experiment of serum was opti-mized by adjusting the conditions of sample pretreatment and silver nitrate staining. Results An ideal 2-DE experiment method with higher repetition was established. The number of protein spots and the resolution were both increased,and this method was compatible with MS analysis. Conclusion After the adjustments and optimizations,an ideal method of 2-DE technique for serum is established,which has a high reference value for the relevant researches,and lays an experimental foundation for the search,analysis as well as identification of the disease related proteins in serum.
