Effect of NVP-AAM077 and Ro25-6981 on hippocampal neuronal injury and brain derived neurotrophic factor expression in global cerebral ischemic mice
- VernacularTitle:NVP-AAM077和Ro25-6981对全脑缺血小鼠海马神经元损伤及BDNF表达的影响
- Author:
Yuanshou CHEN
;
Xiaomei LUO
;
Min CHEN
;
Chi ZHANG
- Publication Type:Journal Article
- Keywords:
NVP-AAM077;
Ro25-6981;
NMDA recep- tor;
transient global ischemia;
brain derived neurotrophic factor;
hippocampus
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The aim of the present study is to in-vestigate the effect of the specific antagonist NVP-AAM077 and Ro25-6981 of NMDA receptor subunit NR2A and NR2B on global cerebral ischemia-induced neuronal injury in the CA1 region. Methods Male C57BL/6 mice subjected to global ischemia by the three-vessel occlusion( 3-VO) method,were divided into four groups: sham-operated group,ischemia/reperfusion control group,NVP-AAM077 treatment group,and Ro25-6981 treatment group. Hippocampal sections were processed for Fluoro-Jade B staining to detect degenerating neurons and for Nissl staining to identify surviving neurons. The expression of brain derived neurotrophic factor( BDNF) were measured by Western blot. Results ① Transient brain ischemia induced selective and delayed neuron death in the CA1 region of the hippocampus at 12-minute ischemia after reperfusion 3 days. NR2A subtype specific antagonist NVP-AAM077 enhanced neuronal death after transient global ischemia ( P
