Effects of matrine on apoptosis of human ovarian cancer cell line SKOV_3 and its mechanism
- VernacularTitle:苦参碱诱导卵巢癌SKOV_3细胞凋亡的机制研究
- Author:
Qishuai GUO
;
Xi HUANG
;
Shaolin LI
- Publication Type:Journal Article
- Keywords:
matrine;
ovarian cancer;
cell proliferation;
apoptosis;
survivin;
Caspase-3
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of matrine( Mat) on the apoptosis of ovarian cancer SKOV3 cells and its related mechanism. Methods SKOV3 cells were treated with different concentrations( 0. 4,0. 8,1. 2,1. 6, 2. 0 g?L -1) of Mat for 24,48,72 h. The inhibitory rate of mat on SKOV3 cell proliferation was determined by MTT assay,and cell apoptosis was evaluated by flow cytometry ( FCM) ; the expression of survivin mRNA was measured by RT-PCR and the expression of survivin and Caspase-3 protein was measured by Western blot. Results Different concentrations of Mat had certain inhibitory effect on SKOV3 cell proliferation,in a dose-and tine-dependent manner. The half maximal inhibitory concentration ( IC50 ) of 24,48,72 h were 3. 38,1. 57,1. 13 g?L -1 respectively; the cell apoptotic rate of treated with 0. 8,1. 6 g?L -1 Mat for 48 h, was( 11. 44 ? 0. 56) % ,( 17. 68 ? 0. 98) % respective-ly,which had statistical significance compared with the control group( 4. 85 ? 0. 31) % ( P
