Effects of ZX-5 on the expression and activity of NOSs
- VernacularTitle:ZX-5对一氧化氮合酶表达和活性的调节作用
- Author:
Jin WEI
;
Li PAN
;
Yihua ZHANG
;
Hanmei XU
- Publication Type:Journal Article
- Keywords:
ZX-5;
age-related macular degeneration ( AMD);
eNOS;
iNOS;
nNOS;
nitric oxide ( NO)
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate effects of ZX-5 on the expression and activity of nitric oxide synthase ( NOS) in endothelial cells,and to confirm which kind of NOS increases NO production and promote choroidal blood flow in ZX-5 -induced HUVECs. Methods HUVECs ( human umbilical vein endothelial cells) were used to determine the expression of eNOS,iNOS and nNOS by Western blot; the activities of NOS were investigated by the corresponding kit. Results ( R,R) ZX-5 upregulated eNOS expression and increased NO production; ( S,S) ZX-5 upregulated iNOS expression and slightly increased NO release. Conclusions ( R,R) ZX-5 promotes choroidal blood flow via upregulating eNOS expression and activity and promoting NO production; the compound may be used for the prevention and treatment of age-related macular degeneration in the elderly.
