Effects of Wortmannin combined with mitomycin C on proliferation and apoptosis of HepG-2 cells
- VernacularTitle:渥曼青霉素联合丝裂霉素对人肝癌HepG-2细胞增殖和凋亡的影响
- Author:
Jian ZHANG
;
Honglin WANG
;
Qian ZHANG
;
Yuan ZHAO
- Publication Type:Journal Article
- Keywords:
liver cancer,Wortmannin,mitomycin C;
apoptosis;
PI3K/Akt signaling pathway
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Wortmannin combined with mitomycin C (MMC) on the proliferation,apoptosis and expressions of related genes Cyclin D1 and Bad in liver cancer cell line HepG-2. Methods HepG-2 cells (104/ml) were treated with 0,25,50,100,200 or 400 nmol/L Wortmannin,0,1.75,3.75,7.5,15 or 30 nmol/L MMC,or combined concentrations (0,26.875,53.75,107.5,215 or 430 nmol/L) respectively. Cell suppression rate were detected by MTT assay. Nucleus apoptosis of Hep-G-2 cells were examined by Hochest 33258 fluorescent staining. Change of cell cycle and apoptosis in HepG-2 cells were detected by flow cytometry (FCM). Expressions of PI3K/Akt signaling transduction pathway related genes and proteins were tested by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Results The inhibition ratio of cell proliferation was significant higher in combined groups than in either single groups (P
