Effects of procyanidins on apoptosis and the expression of apoptosis-related proteins in brain of experimental rats with intracerebral hemorrhage
- VernacularTitle:原花青素对实验性脑出血大鼠脑组织细胞凋亡及凋亡相关蛋白表达的影响
- Author:
Yu GAO
;
Zhi DONG
;
Lu XU
- Publication Type:Journal Article
- Keywords:
cerebral hemorrhage;
procyanidins;
apoptosis;
bax;
bcl-2;
antioxidation
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of procyanidins on apoptosis in brain of experimental rats with intracerebral hemorrhage(ICH) and its possible mechanisms.Method Rat intracerebral hemorrhage model was established by injecting collagenase into the brain caudate nucleus.Fifty-four health Sprague-Dawley rats were divided into six groups randomly: sham operation group,model group of intracerebral hemorrhage,treatment groups with procyanidins at doses of 50 mg?kg~-1,100 mg?kg~-1 and 200 mg?kg~-1,and positive control group with nimodipine at dose of 10 mg?kg~-1.Twentyfour hours after operation,the content of glutathione(GSH) and activity of catalase(CAT) were measured.The levels of bax and bcl-2 were detected by method of immunohistochemistry,and the apoptosis in brain tissues was detected by method of TUNEL.Result In procyanidins groups(50,100 and 200 mg?kg~-1),the content of GSH and the activity of CAT were significantly higher than those in ICH group(P
