Effects of diazoxide on expression of Bcl-2 following oxygen-glucose deprivation in PC12 cell
- VernacularTitle:二氮嗪对氧糖剥夺后PC12细胞凋亡及对Bcl-2蛋白的影响
- Author:
Zhanqiang WANG
;
Chunhong JIA
;
Yang LU
;
Danyang ZHAO
;
Quancai WANG
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
PC12;
ATP sensitive potassium channel;
apoptosis;
Bcl-2;
diazoxide;
5-HD
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of diazoxide on the apoptosis of PC12 cells induced by oxygen-glucose deprivation(OGD)and expression of Bcl-2 protein.Methods Cultured PC12 cells,treated with OGD,diazoxide and 5-HD,were divided into A(control group),B(OGD group),C(OGD+diazoxide group)and D(OGD+diazoxide+5-HD group).Neuronal apoptosis was detected by Annexin V-FITC/PI double-dyed flow cytometry,and the expression of Bcl-2 protein was detected by immunofluorescence and Western blot.Results The number of apoptotic PC12 cells increased after OGD in B,C,D group.C group and B,D groups were significantly different(P
