Lentivirus vector-mediated enhanced green fluorescence protein transfection on in vitro corneal epithelial cells of rabbits
- VernacularTitle:慢病毒载体介导增强型绿色荧光蛋白转导兔角膜上皮细胞的体外实验研究
- Author:
Jidong WANG
;
Zhiyu DU
;
Fen LAN
- Publication Type:Journal Article
- Keywords:
lentivirus;
gene transfection;
corneal epithelial cell;
effectiveness;
in vitro
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of lentivirus vector-medicated enhanced green fluorescence protein (EGFP) transfection on in vitro corneal epithelial cells.Methods Primary corneal epithelial cells of rabbits were cultured and transfected with lentivirus vector into different multiplicity of infection (MOI) in order to find the best transfection dose.After 24,48,72,and 96 h of transfection,expression of EGFP was observed under microscope and detected by RT-PCR.The transfection rate of corneal epithelial cells was calculated in different MOI.Results EGFP was expressed in corneal epithelial cells 48 h after transfection,which increased with the increasing transfection time.The transfection rate of corneal epithelial cells was 4.5%?0.6%,30.4%?0.9%,51.9%?1.4%,and 75.4%?0.7%,respectively,when MOI was 1,10,50,and 100(P
