Inhibitory effect of mycophenolate mofetil on expression of TGF-? in pulmonary fibroblasts and its application value
- VernacularTitle:霉酚酸酯抑制转化生长因子-?在肺成纤维细胞中的表达及应用价值
- Author:
Ying WANG
;
Yue WAN
;
Bing TAN
;
Yong JIANG
;
Yu BAI
;
Yongzhong WU
- Publication Type:Journal Article
- Keywords:
mycophenolate mofetil;
TGF-?;
WI26 cell
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of mycophenolate mofetil (MMF) on expression of TGF-? in pulmonary fibroblasts (WI26 cells) and its application value.Methods Pulmonary fibroblasts were divided into control group,MMF group,TGF-? group,and MMF+TGF-? group,after routine culture.Expression of TGF-?-induced COL1A1 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).Expression of COL1 proteins was detected by Western blot analysis and chloramphenicol acetyl transferase assay,respectively.Difference in contractility of collagen fibers and migration of cells was observed in collagen matrix contraction test and cell scratch test.Results The expression level of COL1A1 mRNA was higher in MMF group than in control group 24 and 48 h after treatment with MMF (77.0%?2.9% vs 38.0%?3.7%),and was lower in MMF group than in control group 24 and 48 h after treatment with TGF-? and MMF+ TGF-? (134.0%?3.1% vs 189.0%?2.4%,and 95.0%?2.7% vs 71.0%?3.3%,P
