Difference of ?-tubulin and aurora-A expression in human cervical cancer and H8 cells
- VernacularTitle:人宫颈癌细胞和永生化细胞的细胞中心体?-tubulin和Aurora-A激酶表达的差异分析
- Author:
Jingxian WU
;
Yong ZHAO
- Publication Type:Journal Article
- Keywords:
cervical carcinoma;
immortalization;
centrosome;
?-tubulin;
aurora-A
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare and analyze the difference of ?-tubulin and aurora-A expression in human cervical cancer cells (CasKi ) and immortalized human cervical squamous H8 cells with positive HPV16 E6E7.Methods Difference of ?-tubulin and aurora-A expression in CasKi and H8 cells was analyzed by showing the fluorescence intensity of ?-tubulin with indirect immunofluorescence.Expression level of aurora-A mRNA was detected by RT-PCR.Expression level of ?-tubulin and aurora-A in CasKi and H8 cells was semi-quantitatively analyzed by Western blot.Results The immunofluorescence signal of ?-tubulin was stronger in Caski cells than in H8 cells (57.78?3.13 vs 37.37?2.37,P
