Effect of electrical stimulus at a low physiological frequency on expression of myosin heavy chain in genioglossus of rabbits with chronic hypoxia
- VernacularTitle:低频复合生理频率电刺激对慢性低压缺氧兔颏舌肌肌球蛋白重链表达的影响
- Author:
Ni ZHANG
;
Xi LIU
;
Gang LIU
- Publication Type:Journal Article
- Keywords:
chronic electrical stimulus;
genioglossus;
subtypes of myosin heavy chain
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of chronic electrical stimulus at a low physiological frequency on the expression of myosin heavy chain (MHC) in genioglossus of rabbits with chronic hypoxia.Methods Twenty-four adult rabbits,weighing 2.3?0.1kg,were randomized into control group (A),chronic hypoxia group (B),10 Hz electrical stimulus group (C),and 10+40 Hz electrical stimulus group (D).Rabbits in groups C and D received electrical stimulus of genioglossus at a frequency of 10 Hz and 10+40 Hz,respectively,while those in group B received no electrical stimulus,after they were placed in a hypobaric hypoxia cabin,8 h a day for 5 weeks,with free access to food and water.Expression of MHC in genioglossus of rabbits in the 4 groups was detected by SDS-PAGE and semi-quantitative inverse transcription RT-PCR,respectively.Results The expression level of MHCⅠand MHCⅠmRNA was significantly lower while that of MHCⅡa was significantly higher in group B than in group A [(0.69?0.07)% vs (17.48?4.00)%,(0.69?0.07)% vs (1.05?0.06)%,and (79.89?5.09)% vs (73.33?4.17)%,P
