Up-regulation of TLR4 in the gut of rats after traumatic brain injury
- VernacularTitle:创伤性脑损伤后小鼠肠道Toll样受体4的表达
- Author:
Ke DING
;
Handong WANG
;
Huilin CHENG
;
Bo WU
;
Chunhua HANG
;
Jixin SHI
- Publication Type:Journal Article
- Keywords:
Toll like receptor 4;
Traumatic brain injury;
Gut mucosal injury
- From:Parenteral & Enteral Nutrition
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The aim of the current study was to explore the alterations of TLR4 after traumatic brain injury. Methods: 20 Male ICR rats were randomly divided into four groups(5 rats each group)including control group without brain injury and traumatic brain injury groups(4,24 hours and 7 days).All rats were decapitated at corresponding time point and mid-jejunum samples were taken.The intestinal mucosa structure was detected by histopathological examination.Immunohistochemistry and realtime RNA were used for detection of TLR4 expression in ileum samples.Results: After traumatic brain injury,the histopatholocal alterations of gut mucosa occurred at 4 hours and progressed to a serious state.Compared to control group,TLR4 was significantly up-regulated on the endothelia of microvessels in villous interstitium and lamina propria by 4 h following TBI and maximally expressed at 24 h post-injury.The endothelial TLR4 immunoreactivity in ileum mucosa still remained strong on 7 d post-injury.There was a very low mRNA expression of TLR4 in the gut of control rats and significantly increased at 4 h following TBI(P
