Establishing Quality Control Ranges for Antimicrobial Susceptibility Testing of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus: A Cornerstone to Develop Reference Strains for Korean Clinical Microbiology Laboratories.
10.3343/alm.2015.35.6.635
- Author:
Sung Kuk HONG
1
;
Seung Jun CHOI
;
Saeam SHIN
;
Wonmok LEE
;
Naina PINTO
;
Nari SHIN
;
Kwangjun LEE
;
Seong Geun HONG
;
Young Ah KIM
;
Hyukmin LEE
;
Heejung KIM
;
Wonkeun SONG
;
Sun Hwa LEE
;
Dongeun YONG
;
Kyungwon LEE
;
Yunsop CHONG
Author Information
1. Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea. deyong@yuhs.ac
- Publication Type:Brief Communication ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Agar dilution test;
Antimicrobial susceptibility test;
Clinical microbiology laboratory;
Disk diffusion test;
Quality control;
Reference strain
- MeSH:
Anti-Bacterial Agents/*pharmacology;
Asian Continental Ancestry Group;
Escherichia coli/*drug effects;
Humans;
Laboratories;
Microbial Sensitivity Tests/*methods;
Pseudomonas aeruginosa/*drug effects;
Quality Control;
Reference Values;
Republic of Korea;
Staining and Labeling;
Staphylococcus aureus/*drug effects
- From:Annals of Laboratory Medicine
2015;35(6):635-638
- CountryRepublic of Korea
- Language:English
-
Abstract:
Quality control (QC) processes are being performed in the majority of clinical microbiology laboratories to ensure the performance of microbial identification and antimicrobial susceptibility testing by using ATCC strains. To obtain these ATCC strains, some inconveniences are encountered concerning the purchase cost of the strains and the shipping time required. This study was focused on constructing a database of reference strains for QC processes using domestic bacterial strains, concentrating primarily on antimicrobial susceptibility testing. Three strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) that showed legible results in preliminary testing were selected. The minimal inhibitory concentrations (MICs) and zone diameters (ZDs) of eight antimicrobials for each strain were determined according to the CLSI M23. All resulting MIC and ZD ranges included at least 95% of the data. The ZD QC ranges obtained by using the CLSI method were less than 12 mm, and the MIC QC ranges extended no more than five dilutions. This study is a preliminary attempt to construct a bank of Korean QC strains. With further studies, a positive outcome toward cost and time reduction can be anticipated.
