Effect of Yukuiqing on expression of connective tissue growth factor in human real mesangial cells incubated with AGEs
- VernacularTitle:玉葵清对AGEs干预的人肾小球系膜细胞CTGF表达的影响
- Author:
Bingquan YANG
;
Zilin SUN
;
Jiangyi YU
;
Feng GAO
;
Su LIU
- Publication Type:Journal Article
- Keywords:
Yukuiqing;
Chinese herbal medicine serum pharmacologic assay;
Glycosylation end products;
Human mesangial cells;
Connective tissue growth factor
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Yukuiqing (YKQ) on the expression of connective tissue growth factor (CTGF) in cultured human renal mesangial cells (HRMC) incubated with AGEs. Methods:The HRMCs were incubated with AGEs (200 ?g/mL) and 1.25% YKQ which was prepared by Chinese herbal medicine serum pharmacological approach for 0,8,16,24,48 and 72h or different concentrations (0.313%,0.625% and 1.25%) of YKQ for 48h,respectively,which were incubated with DMEM and rat serum (RS) as the control. CTGF mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western Blotting respectively. Results:In Yukuiqing group,the expression of CTGF mRNA and protein decreased significantly compared with control groups (P
