Regulation of transcription of hypoxia-inducible factor-1? on telomerase reverse transcriptase in the pulmonary arterial smooth muscle cells
- VernacularTitle:缺氧诱导因子-1?对肺动脉平滑肌细胞端粒酶反转录酶的转录调节作用
- Author:
Jingchun SONG
;
Shan JIANG
;
Guoming HUANG
;
Xiaowen TU
;
Zhongru DING
- Publication Type:Journal Article
- Keywords:
hypoxia-inducible factor 1,alpha subunit;
telomerase;
myocytes,smooth muscle
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of hypoxia-inducible factor-1? (HIF-1?) in the expression of telomerase reverse transcriptase (TERT) in the pulmonary arterial smooth muscle cells (PASMCs) under anoxia.Methods HIF-1? and TERT mRNA in the PASMCs were examined by RT-PCR with the presence of HIF-1? inducers (CoCl_2,DFX),inhibitor (FAS,CBZ-LLL) and oligodeoxynucleotides,or under anoxia.HIF-1? protein in the PASMCs was determined by Western blotting.Results Expressions of HIF-1? and TERT mRNA significantly up-regulated under anoxia (0.59?0.07,0.60?0.06) compared with that in control group ( 0.11? 0.02,0.1?0.01,P0.05).Antisense oligodeoxynucleotides invalidated CoCl_2-induced increase in TERT mRNA levels (0.45?0.04) compared with CoCl_2 group (0.95?0.08,P
